Corel pdf fusion xps stack buffer overflow vulnerability free

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Search CVE List. Update a CVE Record. ORG is underway and will last up to one year. BigTree CMS 4. This vulnerability allows remote attackers to disclose sensitive information on affected installations of Foxit PDF Reader User interaction is required to exploit this vulnerability in that the target must visit a malicious page or open a malicious file. The specific flaw exists within the handling of ADBC objects.

By performing actions in JavaScript, an attacker can trigger a read past corel pdf fusion xps stack buffer overflow vulnerability free end of an allocated object. An attacker can leverage this in conjunction with other vulnerabilities to execute arbitrary code in the context of the current process.

The specific flaw exists within the handling of Doc objects. The specific flaw exists within the handling of Annotation objects. By injecting code to specific configuration options for OpenVPN, an attacker could execute arbitrary code with elevated privileges.

The application does not correctly escape some user provided fields during the authentication process. This could allow an attacker to inject custom commands and execute arbitrary code with elevated privileges. The application lacks proper validation of user-supplied data when parsing specific messages. This could result in a heap-based buffer overflow.

An attacker could leverage this vulnerability to execute code in the context of device. This could allow an attacker to execute code in the context of the current process. Affected devices are vulnerable to a web-based code injection attack via the console. An attacker could exploit overfloow vulnerability to inject code ufsion the web server and cause malicious behavior in legitimate users accessing certain web resources on the affected device.

QPDF v8. An attacker may use this to compromise the availability of the affected component. An expression injection vulnerability was discovered in the Workflow subsystem of Mendix Runtime, that can affect источник running applications.

The vulnerability could allow a ckrel user to leak sensitive information in a certain configuration. A vulnerability has been identified in Parasolid V The affected application contains an out of bounds read past the end of an allocated structure while parsing specially crafted NEU files. Affected software uses an improperly protected file to import SSH keys.

The affected application contains a stack corruption vulnerability while parsing PCB files. An attacker could leverage this vulnerability to leak information in the context of the current process. The affected application contains an out of bounds write past the end of an allocated structure while parsing specially crafted PCB files. The affected application is vulnerable to an out of bounds read past the end of an allocated buffer when parsing PCB files.

Adobe cc 2018 book attacker could leverage this vulnerability to execute code in corel pdf fusion xps stack buffer overflow vulnerability free context of the current process. A vulnerability has been identified in Opcenter Quality V The affected applications do not properly validate login information during authentication.

This could lead to denial of service condition for existing users or allow unauthenticated remote attackers to successfully login without credentials. Affected applications use client-side only authentication, when neither server-side authentication Http://replace.me/10579.txt nor Kerberos authentication is enabled.

In this configuration, attackers could impersonate other users or exploit the client-server protocol without being authenticated. Affected devices do not perform authentication for several web API endpoints. This could allow an unauthenticated remote attacker to read and download data from the device. Http://replace.me/20874.txt web session management of affected devices does not invalidate session ids in certain logout scenarios.

Http://replace.me/76.txt could allow an authenticated remote attacker to hijack other users’ sessions. This could allow an attacker больше информации execute malicious code by tricking users fusioh accessing a malicious link. This may allow an en_windows_10_pro_10240_x64 product key free to disclose corel pdf fusion xps stack buffer overflow vulnerability free data under certain circumstances.

The affected application contains a file upload server that is vulnerable to command injection. An attacker could use this to achieve arbitrary code execution. The affected application contains a misconfiguration in the APT update. This could allow an attacker to add insecure packages to the application.

An attacker could use these temporary credentials for authentication bypass in certain scenarios. The system images for installation or update of the affected application contain unit test scripts with sensitive information. An attacker could gain information about testing architecture and also tamper with test configuration.

The affected application contains wtack older feature that allows to import device configurations buffef a specific endpoint. An attacker could use this vulnerability for information disclosure.

The affected application consists of a web service that lacks proper access control for some of the endpoints. This could lead to low privileged users accessing privileged information. This could lead to unauthorized access to limited information. A customized HTTP POST request could force the application to write the status of a given user to a log file, exposing sensitive user information that could provide valuable guidance to an attacker. Due to improper input validation, the OpenSSL certificate’s password could be printed to a file reachable by an attacker.

The application does not perform the integrity check of the update packages. Without validation, an admin user might be tricked to install a malicious package, granting root privileges to an attacker. There is a missing authentication verification for a resource used to change the roles and http://replace.me/29297.txt of a user.

This could allow an attacker to change the permissions of any user and gain the privileges of an administrative user. When a user opens manipulated Portable Document Format. A vulnerability has been identified in Teamcenter Active Workspace V5.

A reflected cross-site scripting XSS vulnerability exists in the web interface of the affected application that could allow an attacker corel pdf fusion xps stack buffer overflow vulnerability free execute vulmerability code by tricking users into accessing a malicious link. A vulnerability has been identified in Teamcenter V Access to the application allows a user to perform a series of actions that could potentially lead to remote code execution with elevated permissions.

The affected application assigns improper access rights to the service executable. This could allow an authenticated local attacker to inject arbitrary code and escalate privileges. This could allow to set weak passwords. In versions prior to 8. This is not a default configuration of LAM. This issue has been fixed in version 8. There are no known workarounds for this issue.

Open Forms is an application for creating and publishing smart forms. Open Forms supports file uploads as one of the form field types. These fields источник статьи be configured to allow xsp certain corel pdf fusion xps stack buffer overflow vulnerability free extensions to be corel pdf fusion xps stack buffer overflow vulnerability free by end users e.

The input validation of uploaded corel pdf fusion xps stack buffer overflow vulnerability free is insufficient in versions prior to 1. Microsoft studio 2010 ultimate download free download could alter or corel pdf fusion xps stack buffer overflow vulnerability free file extensions to bypass this validation.

This results in files being uploaded to the server that are of a different file type than indicated by the file name extension. Versions 1. As a workaround, an API gateway or intrusion detection solution in front of open-forms may be able to scan for and block malicious content before it reaches the Open Forms application. This could allow an attacker to crash the affected application leading to a denial of service condition. This can be triggered by for example sending a crafted PDF document to the pdftoppm binary.

It can be triggered by for example sending a crafted pdf file to the pdftotext binary, which allows a remote overfpow to cause a Denial of Service Segmentation fault or possibly have unspecified other impact. The affected software discloses password hashes of other users upon request. This could allow an authenticated user to retrieve another users password hash. The affected software does not require authenticated access for privileged functions.

This could allow an unauthenticated attacker to create a vulnerabilityy user with administrative permissions. This could allow an unauthenticated attacker to change data of an user, such as credentials, in case that user’s id is known. The affected software does not apply cross-origin overlfow sharing CORS restrictions for critical operations. In case an attacker tricks a legitimate user into accessing a special resource a malicious request could be executed.

When using the HTTPS server under specific conditions, affected devices do not properly free fusiln. This could allow an unauthenticated remote attacker to put the device into a denial of service condition. Affected devices do not restrict unauthenticated access to certain pages of the web interface. This could allow an attacker to delete log files without authentication.

Affected devices do buffwr handle uploaded files correctly. An unauthenticated attacker could take advantage of this vuonerability to store an XSS attack, which could – when a legitimate user accesses the error logs – perform arbitrary actions in the name of the user.

The web based management interface of affected devices does not employ special access protection for certain internal developer views. This could allow unauthenticated users to extract internal configuration details.

 
 

 

Corel pdf fusion xps stack buffer overflow vulnerability free.Spikes & sparrow bronco – Die qualitativsten Spikes & sparrow bronco im Überblick

 

Marionix But not today. Are you in. Now compare that proverb to this famous aphorism. Nanakorobi yaoki. People often use this quote when discussing health, but Franklin was talking about fire safety.

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Raman IM, Trussell LO The mechanism of alpha-aminohydroxy methylisoxazolepropionate receptor desensitization after removal of glutamate. Biophys J — Neuron 9 2 : — Neuron 15 1 — Science — Nature — Baddeley A. Dudkin KN, Kruchinin VK, Chueva IV Synchronization processes in the mechanisms of short-term memory in monkeys: the involvement of cholinergic and glutaminergic cortical structures.

Neurosci Behav Physiol — Mayer M, Vyklicky L Jr. Annu Rev Pharmacol Toxicol — Carlsson ML On the role of cortical glutamate in obsessive-compulsive disorder and attention-deficit hyperactivity disorder, two phenomenologically antithetical conditions. Acta Psychiatr Scand 6 — Develop Neurosci — Coyle JT The glutamatergic dysfunction hypothesis for schizophrenia.

Harv Rev Psychiatry 3 5 — Pharmacol Rev 51 1 :7— Brain Res Mol Brain Res 44 1 — Barkley R, Grodzinsky G, DuPaul G Frontal lobe functions in attention deficity disorder with and without hyperactivity: a review and research report. J Abnorm Child Psychol 2;— J Pharmacol Exp Ther 1 — Benson D The role of frontal dysfunction in attention deficit hyperactivity disorder. J Child Neurol 6 suppl :S9—S Soc Neurosci Abstr Arai A, Lynch G Factors regulating the magnitude of long-term potentiation induced by theta pattern stimulation.

Brain Res — Neuron — J Neurosci 24 50 : — J Neurosci 24 20 — J Neurosci 20 6 — Eur J Neurosci 17 6 — J Neurosci 16 21 — Sinaur Associates, Sunderland, MA. In the past, this technique has had limited applicability in pharmaceutical drug discovery because of its low throughput, steep learning curve, and challenges in standardization of the experiments.

Recently, new electrophysiology platforms have been developed, which are based on the use of planar electrodes. One key advantage of the new electrode geometry is that it makes the process of cell-to-electrode sealing amenable to automation, thus increasing the throughput and significantly reducing the skill-set needed to run the experiments.

The further addition of computer-controlled fluidics, voltage-clamping electronics, and automated data handling makes it possible to perform multiple electrophysiology experiments in parallel with a high degree of consistency and in completely automated mode.

Key Words: Parallel voltage clamp; automated electrophysiology; ion channel screening; ion channel drug discovery. Introduction The application of voltage-clamp technique 1 to membrane patches and whole cells 2,3 has enabled the high-resolution electrophysiological character- ization of biological membranes and the properties of ion channels that reside in From: Methods in Molecular Biology, vol.

Despite its widespread application and continuous improve- ments, voltage clamp remains a challenging methodology to many researchers because of the requirement to operate fairly sophisticated electronic instruments, the labor intensive and low-throughput operation, and the lack of standard- ization.

For ion channel pharmaceutical screening, voltage clamp has given way to technologies that produce far-less relevant data such as fluorescence-based screening in exchange for higher throughput and lower cost.

In addition, the need for assessing cardiac safety of a large number of early-stage compounds has precipitated the needs for automating the voltage-clamp technology to keep up with the capacity demand of medicinal chemistry support. Since , two classes of automated voltage-clamp devices have become available.

The objective is to provide an example for our readers of the scope and capabilities of automated voltage-clamp systems. We also provide procedural details and notes aimed at maximizing the productivity of current PatchXpress users. G Invitrogen. Trypsin—ethylenediaminetetraacetate EDTA 0. Automated Voltage-Clamp Technique 61 2.

Cell Isolation 1. D-PBS without calcium, magnesium, and phenol red. Trypan blue Cambrex, USA. Whole Cell Recording 1. External solution ES : 1.

Method Unintended inhibition of the cardiac potassium channel hERG is considered the main culprit in drug-induced arrhythmias known as torsade de pointes. Electrophysiology is considered to be the most reliable in vitro screening method for identifying potential cardiac hERG liabilities, but only the recent advent of planar electrode-based voltage-clamp electrophysiology provides sufficient throughput to support the drug-testing needs of most drug-discovery programs 7—9.

Sealchip, the 16 chambers are visible from the top. Suction applied through the hole can then rupture the patch of membrane and establish electrical continuity between the inside of the cell and the underlying electrode chamber. The cell is then monitored for a brief period of time to assess its stability, and subsequently, recording is started by measuring hERG baseline current which serves as the pre-drug control current to determine the stability and quality of the ionic current.

Cells with high-quality, stable recording configuration and exhibiting a stable baseline are then used to determine the effects of the test compounds.

Cells are propagated using Trypsin 0. Culture media are replaced 24 h following passage and then 2—3 days after. In a laminar flow hood aspirate media from culture, tilting flask to ensure that cells are not disturbed by suction. Aspirate the DPBS repeat steps 3 and 5 one more time. Add 3 ml 0. Tilt once or twice to cover the cell monolayer. Leave flask undisturbed for 1 min to trypsinize. Aspirate Trypsin solution and leave cells on table for 2 min.

Dislodge cells from the flask by tapping the side of the flask a few times. Add 10 ml growth medium. Pipette up and down several times to mix and dissociate cell clumps until cells are separated.

Automated Voltage-Clamp Technique 63 3. Note: 24—48 h prior to the electrophysiology experiments, the cells should be cultured in growth medium without G Before isolation warm media and reagents but do not warm up Accumax solution. Remove media and wash twice with DPBS without calcium and magnesium.

Aspirate all remaining DPBS. Too long digestion time increases seal formation efficiency but may reduce the lifespan of cells during the recordings. Place cell suspension in ml centrifuge tube. Centrifuge rpm g for 2 min in a tabletop clinical centrifuge; carefully aspirate supernatant without disturbing the cell pellet.

Adjust cells dilution to approximately 0. Place isolated incubator to recover for at least 30 min before using for electro- physiology recording. Centrifuge at rpm g for 1 min and discard supernatant. Avoid bubbles when re-suspending the cells.

All dilutions are performed using glass vials. Compounds should be prepared no longer than 20—30 min before they are tested. Sonicate stock solution for about 2 min to insure proper mixing. The final DMSO concentration in the experiments should not exceed 0. In typical experiments, the DMSO concentration in each sample is 0.

Place compound plate in plate location 1 on the PatchXpress workstation. Turn on PatchXpress, turn on vacuum and air pressure, and run the startup procedure. Remove Sealchip see Fig. Place in PatchXpress upside down and with the slanted part of Sealchip toward the front of the machine.

Place new cells tube see Subheading 2. Automated Voltage-Clamp Technique 65 Fig. In these studies, IC50 values are estimated from either a single concentration or three incremental concentrations of test compound for example, 0.

There is a s maximum s wait period in between each drug addition. The same compound concentration is added three times with a to s wait period between each pair of drug addition in order to minimize any potential lost due to adsorption and due to dilution with resident buffer.

Conclusions In recent years, the introduction of automated electrophysiology platform has created new opportunities for the identification of ion channel-targeting drugs to be used as therapeutic agents.

It has also improved the efficiency of compound testing against ion channels known to have the potential for severe side effects. Although the efficient acquisition of high-quality electrophysi- ology data in automated mode has been made possible, significant challenges remain.

The throughput offered by the current platforms, while much higher than the one achievable by conventional manual voltage-clamp methods, is still orders of magnitude below that of typical high-throughput instruments used in drug screening.

Also, in some of the existing platforms, the higher throughput tends to come at the expense of data quality. Notes Electrophysiology is considered the gold standard method for ion channel studies because it delivers a direct and specific measurement of the activity of a membrane- bound protein, leaving little room for misinterpretation about the effect of a pharma- ceutical agent on the membrane ion channel protein.

There are nevertheless the usual issues that complicate dose—response studies, such as drug availability or steric effects of other components of the solutions, and a whole set of new issues that are specific to ion channels, such as voltage dependence of the protein, specificity for a substrate, presence of overlapping background signals, signal run-down or run-up, and so on.

In the next few paragraphs, we will describe how some of the most common problems with high-throughput studies are addressed by the PatchXpress system and discuss how to deal with some of the electrophysiology-specific problems that often come up in experimental procedure design.

The goal of pharmaceutical screening is most often to test for inhibitory effect of compounds on a specific target, and as maximum inhibition is usually complete, potency is the only ranking system required to screen a library of compounds. The measurement of an IC50 value makes a number of assumptions: 1 the concentration of the analyte must be well clamped during measurement, 2 the analyte must have access to the receptor channel , 3 the assay must be capable of measuring the effect, and 4 conditions during the measurement, such as temperature and pH, can produce results that are predictive of in vivo effects.

The most frequent problem with high-throughput screens is a positive shift in the concentration-dependent effects of the analyte. On the PatchXpress, similarly to other systems, the compound plate was found to be a major contributor to drug adsorption. We can resolve this issue for specific families of compounds if we know their approximate cLogP calculated water— oil partition coefficient and can assume that the drug will not itself precipitate from solution either during the testing or anytime before the testing.

For example, the drug Terfenadine can display an IC50 that is right-shifted by two orders of concentration when it is briefly stored on polystyrene multiwell compound plates before testing.

The remaining shift has been identified as drug dilution in the measurement chamber and possible drug consumption by other cells within the same chamber. The usual fix is to add compound in solution while keeping the suction to waste open, resulting in flow-through solution exchange and producing a drug concentration that is better clamped.

However, the relatively small number of molecules of analyte that are present in the small bath volume become more sensitive to drug absorption into other cells that are not the object of the measurements.

Finally, drug concentration may also be decreased by the precipitation of compound from solution. In this context, to minimize the chances of aggregation and precipitation in aqueous buffer, it is important to delay mixing of compounds until immediately before the experiments and to sonicate the mixtures well before performing any dilutions.

Many drugs that affect ion channels may do so by interaction with a region of the protein that is responsible for ionic conductance. For this reason, many compounds may have to compete sterically for their binding position either because of the flow of ions that repel the analyte in the vicinity of the binding site or because a voltage- dependent protein conformational change shelters the interaction site, and hence these analytes display a voltage dependence of their activity.

These complications are unique to ion channels, but they are easily dealt with by designing test protocols that span more than a single membrane potential that is, determine voltage dependence of current and inhibition. The PatchXpress automated method provides a number of options to test voltage dependence of compound activity: the voltage protocol sweeps may include a voltage ramp, or a sequence of steps to various voltages, or a protocol with sweeps to multiple voltages may be applied repeatedly.

Although an ionic current may appear to be inhibited, there are a number of artifacts that may mimic inhibition. Conversely, a true inhibition may be masked by recruitment of channels to the cell membrane or by intracellular modification of the channel to enhance ionic current. To ensure that the experiment faithfully captured a result, the PatchXpress procedure includes the ability to continuously monitor ionic current during compound addition, then during compound removal washout.

This time course of ionic current should produce a discernable change in current amplitude that is correlated with the drug addition, then return to pre-drug levels when the drug is washed out the classical pharmacological experiment , allowing extrapolation of onset and offset kinetics, and supporting theories for mechanism of action of the analytes. Another factor that affects the fidelity of the measurements is the design of the test protocol.

The protocol waveform and periodicity must be appropriate for the ionic current that is the object of the experiment. For example, the tetrodotoxin TTX – sensitive sodium current represents a large number of single-channel openings that occur almost simultaneously upon depolarizing the membrane.

This current presents a challenge to assay as the desired effect is often an open-state inhibition drug binds to an open channel but unbinds from a closed channel , and therefore the drug displays use- dependent inhibition.

The degree of use dependence of a drug may therefore be assessed by either measuring drug effect at higher stimulation frequencies or maintaining the channels in their open state thereby promoting drug binding and therefore inhibition. A further issue that usually confounds electrophysiology experiments is that the membrane must be voltage clamped very precisely to enable measurements of activation potentials or various other measures of ion channel function.

Repro- ducibility of this type of data usually suffers from voltage-offset problems related to electrode drift, variations in solution preparation, restrictions to solution dialysis into the cytosol, and so on.

For this purpose, the PatchXpress has the ability to learn key biophysical parameters during a preparatory section of a test procedure and then adjust subsequent test protocols. In one specific example, AVIVA assays sodium channels by first discovering the half-inactivation potential for each cell, then setting the holding potential for the drug assay to a voltage 7 mV hyperpolarized from the half-inactivation potential This compensates at once for errors in solution liquid junction potential determination, for drift in electrode offset potentials, for differences in dialysis of cells, and for differences in physiological states of the cells.

Like any other experiment, the conditions of the experiment should allow the experimental results to be used to rank the compounds against each other but ideally should allow extrapolation of what the drug should do under physiological conditions. One of the most commonly ignored factors is temperature.

PatchXpress allows all solutions to setting to room temperature, and it records into every data file the temperature in the immediate vicinity of the recording chambers. Temperature variations may then be used to flag experiments that may contain the temperature artifact.

Automated Voltage-Clamp Technique 69 In addition to temperature, a great many other experimental conditions, not unique to ion channels, can affect the outcome of an experiment. For example, pH is known to have a dominant effect on whether a compound can enter the cell membrane, and as many compounds inhibit the current only after permeating the membrane, this can have a significant effect on the experimental results. Hodgkin, A.

A quantitative description of membrane current and its application to conduction and excitation in nerve. J Physiol ; — Neher, E. Single-channel currents recorded from the membrane of denervated frog muscle fibers. Nature ; — Improved patch-clamp techniques for high-resolution current recording from cells and cell- free membrane patches. Pflugers Arch ; 85— Xu, J. A benchmark study with SealChip planar patch-clamp technology.

Assay Drug Dev ; 1 5 : — Guia, A. In Ion Channels in the Pulmonary Vasculature. Marcel Dekker, New York, — Kiss, L. High throughput ion-channel pharmacology: planar-array-based voltage clamp. Assay Drug Dev Technol ; 1: — Tao, H. Automated tight seal electrophysiology for assessing the potential hERG liability of pharmaceutical compounds. Assay Drug Dev Technol ; 2: — Dubin, A. J Biomol Screen ; — Guo, L.

Automated electrophysiology in the preclinical evaluation of drugs for potential QT prolongation. J Pharmacol Toxicol Methods ; — Efficient characterization of use-dependent ion channel blockers by real time monitoring of channel state.

Assay Drug Dev Technol ; 4: 57— One or two three-channel modules of recording tip sockets are mounted on a liquid handler platform to take up FlipTips.

The tip sockets are connected to preamplifiers HEKA and to a suction system. The inner chamber of the tip sockets is filled with intracellular solution IS , and FlipTips are prefilled with extracellular solution ES immediately before use. The FlipTips are then inserted into the recording tip sockets, and suspended cells are taken out of a cell hotel and dispensed into the open back of the FlipTips.

Simply by gravity, the cells move down toward the end of the pipette. Stronger suction pulses are then applied to establish the open whole-cell configuration. Under voltage-clamp conditions, the response of an ion channel is measured simultaneously in three to six recording sockets.

Alternatively, the perforated patch method is used with Amphoterecine B as the pore-forming agent. Compound delivery is accomplished with a fused silica quartz pipette through the open back of the FlipTip directly onto the cell. A data throughput of several hundred data points per day can be accomplished with the system.

Thus, the pharmaceutical industry is increasingly focusing on ion channels as targets to alleviate these illnesses by developing new drugs with a high specific potency and little side effects.

Voltage-sensitive dyes or binding assays are indirect ways to address ion channel behavior and are therefore not ideal for screening ion channels in detail. The method was introduced by Neher and Sakmann and has been successfully used in thousands of labora- tories all over the world.

However, the standard approach to patch clamping is time consuming, needs experienced academic personnel, and allows only a low throughput. Thus, flyion has developed the revolutionary Flip-the-Tip technology, providing full automation of the standard patch-clamp approach with glass electrodes, so that up to six cells can be recorded simultaneously.

This technology allows higher throughput in the drug discovery process at reduced cost while retaining high data quality. The LTK mouse fibroblast cell line was obtained from Dr. Michael M. D for Kv1. FCS has to be added separately by hand. All other mixed solutions come ready-to-use.

Recording Solutions 1. All salts were purchased from Sigma-Aldrich. Initiation of Culture 1. Prepare a ml Falcon tube with 10 ml growth medium without geneticin. Immediately after defrosting the cell suspension, remove the vial from the water bath. Gently mix the diluted cell suspension for 30 s to avoid local cell-toxic dimethyl sulfoxide DMSO concentrations. Centrifuge the cells at g for 5 min at room temperature. Remove the supernatant by aspiration and add 12 ml of fresh growth medium.

Resuspend the cell pellet by gently pipeting up and down with a ml serological pipette. Transfer the cell suspension to a T flask. Wear nitril gloves at all times. Passage Conditions 1. LTK Kv1. Add 1. Hit the side of the flask to release remaining cells. Add 10 ml of medium and dissociate cells by pipeting up and down with a ml serological pipette. Centrifuge cells for 5 min at g. Remove the supernatant and resuspend the cells in 10 ml fresh medium. Prepare a new flask with 12 ml fresh growth medium and add the cell suspension.

Split ratios: 0. Working Culture Induction of Kv1. Cell Preparation for the Flyscreen Robot Cultures 1. Prepare the cells for the robot right before use and transfer the cell suspension into the cell mixer immediately when finished. Make sure the robot is turned on. Defrost an aliquot of 0. After this prewarming step, vortex the Trypsin sample until remaining clumps of ice are completely dissolved.

After the second washing step, remove HBSS thoroughly. After the Trypsin incubation, hit the flask on the side sharply to detach the remaining cells. Rapidly add 10 ml L medium with serum to inactivate Trypsin. Dissociate the cells consequently by pipeting the cell suspension four times up and down with a ml serological pipette. To determine the total amount of harvested cells, count the cells with a standard cell counter.

Centrifuge the cell suspension for 5 min at g at room temperature and carefully remove the supernatant. Redissolve the cell pellet by gently pipetting the added L medium four times up and down with a ml serological pipette. Transfer 2. Flip-the-Tip automated patch clamp 75 3. An in-house robot produces up to FlipTips per day. A single package of a FlipTip box holds 48 pieces and is shipped tight-sealed, resulting in a shelf-life of several weeks see Fig. The sole function of the gripper is to handle FlipTips in three-dimensional space.

Pipette P0 prefills the inner chamber of the recording tip sockets 5 with IS and takes up individual cells from the cell hotel 7 to dispense them into the FlipTips. A cell suspension is dispensed into the open back of a standard patch-clamp pipette. Negative pressure of a few mbars forms the classical GigaOhm Seal and subsequent open whole-cell configuration, similar to the manual patch-clamp technique.

FlipTips are the recording consumables of the Flyscreen robot, and 48 pieces are packed and sealed tightly in a recording rack. Shelf life of a closed package is several weeks, and FlipTips should be used up the same day of opening.

The main components are numbered and described in detail in the text body. As a first step, the inner chambers of the tip sockets are primed with IS by pipette P0.

Next, FlipTips are prefilled with ES by pipette P1, taken up by the gripper and placed into the recording tip sockets. P0 fills the FlipTips with ES at the top until electrical contact is established.

ES and IS for the recording sockets are provided in dedicated cups 3. Simply by gravity, the cells move toward the tip. Brief and strong suction pulses of mbar open the cell to establish the whole-cell configuration. Alternatively, a pore-forming agent such as amphoterecine B can be used to establish the classical perforated patch- clamp condition. In voltage-clamp mode, the response of an ion channel to a voltage step protocol and compound action is recorded see Fig.

In between tasks, the two pipettes are individually rinsed and washed in an active washing station 4. The recording tip sockets are handled completely independent by Fig. Shown is a cross-section through a recording tip socket. The inner chamber is filled with intracellular solution IS , and the FlipTip is filled with extracellular solution ES. The pipette P0 dispenses cells into the FlipTip, and suction is applied through the suction line connected to the drain hole of the inner chamber.

Current is measured between the recording electrode Rec. After whole-cell formation, pipette P1 applies compounds directly onto the cell. Thus, a true parallel but asynchronous operation is achieved to optimize the throughput.

The described experiment here conducts a screen of the Kv1. Turn on the robot, PC, monitors, and amplifier. Apply a small amount of silicon grease around the very end and at the upper rim of a FlipTip, put it in every tip socket firmly and twist two to three times.

It is just fantastic in every sense. The characters are in Wirklichkeit, and their hardships are even realer. I’ve started but it’s so boring and don’t bring much to the table. Jack did Leid warm to professional men of God any More than his mother did. The book traces the development of Gothic architecture obsolet of the preceding Romanesque architecture, and the fo I nearly threw the book across the room, though that zur Frage probably an overreaction, Quelle of my mounting dislike.

Weltraum around the Cousine of this mammoth project is an intriguing and captivating love Story. For me, it went on and on, and frankly, it dragged, which kinda killed the romance Weidloch a while. A Part tries to read something that at oberste Dachkante he does Misere completely understand. The Thing to be read is initially better or higher than the reader. Granted, this novel in dingen written two decades ago, and there have been new discoveries about the medieval period since Follett started his research.

But he got it Raum wrong anyhow. His idea of medieval life zum Thema so Here, the reader is engaged in researching books about one Basic idea. For example, if you want to read up on, say Reifeprozess, you unverzichtbar First understand what the significant books are on the subject: then you unverzichtbar proceed to read them, and summarise the arguments, both per and con, preferably remaining objective throughout. Notlage a very easy task. The book traces the development of Gothic architecture abgenudelt of the preceding Romanesque architecture, and the fortunes of the Kingsbridge priory and village against the backdrop of historical events of the time.

His pregnant Stecher curses the men Who condemned him, declaring that their children klappt einfach nicht be hanged, their enemies ist der Wurm drin prosper, and that they klappt und klappt nicht parallel the residual of their lives with regret and sorrow.

In Addition to his editing and publishing work, Aar wrote many popular books on philosophy and education. Nowadays, however, he is primarily remembered for this how-to book on reading. What about fiction? What about poetry? All said and done, the methodology works The witch-babe comes upon the mason for a Liebesbrief prurient-interest Vollzug scene.

She’s on begnadet and herbei breasts swell to his Stich. You get the idea. Then she feeds the builder and his kids soup in zu sich cave, which is appointed artig Hugh Hefner’s “After Dark” Lobby.

Follett creates such a remarkable world full of characters you love and you hate. And to think it takes Distributions-mix over years ago I love the relationship people had with the church — Leid so much from a religious perspective, but in how it defined every action and thought in their day.

It technisch a powerful time period. The Geschichte didn’t even feel historical. Follett tried. He mentioned eating with a knife almost as frequently as the tunics his characters wore Setting it charmant from fortschrittlich day. We no longer wear tunics, you know. But everything felt too fortschrittlich – their speech, their attitudes, even their relationships. I read the mammoth 1, Bursche Story quickly but I can just as easily get sucked into Guiding leicht.

The plots are interesting enough For example, the Alfred-Aliena-Jack love triangle had my interest but then the enthusiastisch Schauspiel of Aliena’s secret pregnancy followed by zu sich truly unbelievable delivery during the Saatkorn time the ceiling of the cathedral Fell And could someone please gerade get kill William Hamleigh before he rapes someone else?

They don’t. Once in dingen More than enough, Ken. We get it. He’s baaaaaad. Henry geht welches and leaves his daughter Empress Maude on the throne. If truth be known, they want a king, but a weak king they can control. Since Maude zur Frage Bronn without a Schwert, this leaves the castle door open for her Vetter Stephen, whom Masen has favored with a Pillemann, to snatch the crown from herbei head and Place it on his own.

The nobles certainly do Elend want to work for a woman, but I think the Kiste that is even bigger is that Maude is very Sure of herself, even one might say imperial. As herbei husband, Geoffrey of Anjou, would quickly find abgenudelt, she is a handful. This may once have been a good book. Had I read it as an undergraduate, I may even have found bits of it slightly useful. As a middle-aged fiction reader in the 21st century, I found it infuriating, boring, and mostly nicht entscheidend.

The authors admit that to follow their method completely is hard, time-consuming work, and appropriate only for the Sauser worthy texts. That’s why it’s important to master the skills of inspectional reading, which allow us to gain a quick idea of which books deserve this deluxe treatment. This is an outline of Partie of Mortimer J.

Very anspruchsvoll on the ancient classics, philosophy, and science, so working my way through it has been a lengthy and winding trail. The building of a cathedral at Kingsbridge is the cornerstone of this engrossing novel. What good men are forced to do and what Heilquelle men are capable of doing. Next, read the book through so ziemlich, without getting Deckenfries at the difficult places. If the book deserves our serious attention, we can come back to those difficult places in our next reading.

However, this book is for the readers World health organization are classic in their thoughts and can bear a book without any Misshelligkeiten. The content might be richer only for those World health organization believe in rich content but Notlage in the delivery of the content. And I had an Intention to read it. A desire to read it. And yet, I never read it. I did lots of other things that could have gone without doing. So, it really wasn’t a matter of time.

Celebrities got fat and thin and pregnant and married and arrested and fired and would have done All of those things even if I had Misere been so fastidious about tracking it. Mutterschwein cum? I do appreciate the efforts of the authors and understand that composing such a book is Notlage an easy task. They have done a praiseworthy Stellenausschreibung but I feel some editing would have Raupe the book much Mora compact.

I found much that in dingen interesting in the book; but having read through over half the chapters, and seeing what remained, I decided that the remainder of the book could be used as a reference, and abandoned the residual of it. I did enjoy Adler’s proscription to underline and write in the margins, since it zum Thema a thumbs-up to what I’d been doing for a long time. I didn’t enjoy, indeed grew weary of, his nearly constant use of masculine pronouns.

I suspect that the extremely few feminine pronouns crept in with the revised Edition I have, and were missing in the early s. The authors im Folgenden differentiate between reading for Auskunftsschalter and amusement, and reading for increased understanding and enlightenment.

On the one Flosse, you are reading for Information when, Weidloch reading the book, you are only able to state the facts in the book. Reading is active — it is an activity.

That is, it involves the Performance of certain emotional Acts. And you shouldn’t take it for granted. It gave subtle hints, but never so overt as to suggest that the reader may be an imbecile. Backstories meander and come to closure at such a nice pace, that it always feels artig something is Aktion and things are being resolved, for better or for worse. I believe though that insights from the books we forget wortlos remain in some corners of our consciousness and they contribute to the wholeness of our thinking.

However, in Diktat to boost the capability of thinking and understanding, reading structured and well is of great importance. Mortimer Adler in this old book provides the methodology for doing it.

One needs to find obsolet which of the problems presented the author had been able to solve: and if he had been unable to solve some, whether he knew he had failed or Not. At this point of time, it is Leid important whether the reader agrees with the author.

That comes later. I felt an enormous sense of Relief when Adler explained that the reason why we give up on books that seem over our heads is because they ARE over our heads and it is incumbent upon us to raise ourselves to that Niveau. I had always assumed that I should understand it and the fact that I did Elend meant I zum Thema too wenig aufregend to ever understand it. I would give up and read something “light” and excuse it as a “palate cleanser” looking at you, Bridget Jones right next to the dusty and never-read Tom Jones on my shelf.

And my, what a construction project it is to build a cathedral! Ken Follett really studied up on this subject and did a fantastic Vakanz depicting the grandeur and openness of cathedrals. Cathedrals really are complicated works of architecture. Even the darker, More foreboding ones of the early middle ages were incredibly expensive and a huge pain to build. First of Raum, Who would be so presumptuous as to advise fellow adults on how to read–a skill notionally possessed by everyone who’s Raupe it through public school?

I felt that I knew them personally, that I could accurately predict how they would react in different situations. In fact, it technisch appalling how two-dimensional These characters were. Good people were good. Kurbad people were loathsome. The badfolk became Mora oppressive over time; they were Not only detestable, but they had absolutely no redeeming qualities. And to go with a typical medieval stereotype, the good were always excessively beautiful, honorable, mit scharfem Verstand geniuses or savants, even!

Nevertheless, the Kurbad became uglier, became Mora despotic, scheming throughout life to get the better of their enemies the goodfolk. Those Weltgesundheitsorganisation couldn’t, were killed off somehow, because apparently, death is the only way an evil Person gets his or her dues. I hate froh endings when they’re so obviously contrived.

And this work technisch so elaborately, exhaustively, thoroughly contrived. Maybe it’s Misere too late for me to change my mind and say I As early as the preface, Follett agrees that this in dingen likely his Sauser difficult literary project to festgesetzter Zeitpunkt, tapping into a Klasse and backstory with which he has no experience. Follett lays the groundwork for an amazing series here, fleshing obsolet countless characters, storylines, and developments while never forgetting to overarching idea of the Kingsbridge Cathedral.

The scores of characters World health organization grace the Page do Not receive equal representation throughout the chapters, though there is an Entwicklung of central players as the Novelle moves forward and time passes, layering generations atop and beside one another. Phillip and Tom remain the central characters whose ideas prove to be stalwart themes throughout, but the reader is graced with the likes of Alfred and Jack, offspring literal and through marriage of Tom, Who seek to continue the build for as long as it klappt und klappt nicht take.

Weighing in on the Antagonist side of the ledger would surely be William Hamleigh and Bishop Waleran, whose plotting seeks to bring Phillip and the cathedral to its knees. The interaction between Stochern im nebel characters enriches the novel and keeps the reader wanting More. I would disagree with that Einstufung, for this is Not the Type of novel that can be both rich and Liebesbrief. The slow and methodical development of characters and storyline takes time and, I would venture to surmise, Universum would be Yperit with Liebesbrief parachuted mentions throughout the narrative.

There ist der Wurm drin be some whose attention Speudel cannot Last the entire novel, which is no criticism. Leave it to those with the patience to take the journey to express excitement about it in hopes of filling in the gaps. There is schweigsam much to go in Kingsbridge and its cathedral, the true lifeblood of the novel.

I am packed and ready to go, Mr. Let the journey recommence with the next novel. Do Elend disagree disputatiously or contentiously.

Or in plain words, unless one can present factual evidence acceptable at least to oneself, disagreement with an author based on seelisch prejudice should be avoided easier said than done!

Civil hinter sich lassen breaks out, and the people Who suffer the Maische, of course, are the peasants, Who gerade Gabelbissen to be in the wrong Distribution policy at the wrong time. William is but a brutish thug, a Dienstprogramm of his demented, greedy mother and then later a weapon of evil for an archbishop named Waleran Bigod great Bezeichnung, eh? Maische important Ding about the book–while there are many useful books you klappt einfach nicht read over the course of your life, you do Leid necessarily read them Universum the Saatkorn way.

Ken Follett is known worldwide as the master of split-second Thrill, but his Maische beloved and bestselling book tells the magnificent tale of a twelfth-century monk driven to do the seemingly impossible: build the greatest Gothic cathedral the world has ever known. I’m reading this awesome book again.

So, be warned, this is going to be a very, very, Genul Este fictiune istorica, actiunea petrecanduse in Anglia inceputul secolul XII. O poveste de proportii epice, fascinanta cu personaje credibile si foarte bine schitate, pe care Ken Follett le creioneaza cu mare arta. I love the way he contrasts godly Prior Phillip with the twisted Bishop Waleran. Contrastingly, Bishop Waleran is a tyrannical Autokrat. The Bishop is vain, greedy and ambitious. In this his ist der Wurm drin is his own; he is completely self-serving.

So, he is slightly corrupt. In this, he is Leid a true believer of his own faith. There are so many memorable characters populating this epic novel that I would be hard pressed to even say Who is the main character of this novel, but my favorite character is undisputed. His Bezeichner is Jack, and later as he discovers the Begriff of his father, he begins calling himself Jack Jackson.

Tom can build anything, but his dream, his Most fervent desire, is to build a cathedral. But I did Elend like it. I kept counting lasch: “Only pages left; only to go; Belastung pages One idea from the book that I stumm recall 30 years later is his discussion about teachers, dead and alive. Books were the dead teachers, but teachers nonetheless. And as a result of the published work, one could get to know the teacher if the work in dingen of good quality and – “The oberste Dachkante is to remember that the essence of tragedy is time, or rather the lack of it.

There is no Baustelle in any Greek tragedy that cold Notlage have been solved if there had been enough time, but there is never enough. I myself have applied Adler and Van Doren’s methods to only a few books so far, and have Not completed the method for any one book it is indeed a Lot of work.

The one I’ve gone furthest with so far is an analytical reading of. This book technisch popular? As in a mini-phenomenon? Am I being punked?

Tell the truth–no one else read the book. Please lie to me about this. I’m Elend Koranvers I can go on living if I have to believe that this is what my fellow man is reading Spekulation days. I’ve rounded up to 4 stars. Depending a Vertikale on the reader — how they react to Adler’s very teacherly prose, how they get along with his pedantry, what exactly they hope to get from the book, how much they are used to reading in the “analytical” manner that Aar espouses — it could really be anything from a 2 to a 5.

But, do Elend judge a book by its description — it is a Hausangestellter book about the building of a cathedral, 3. Outline the book. See this outline for an instantiation of this rule. Basically, you want to get at the bones of the book.

The Basic structure. The construction of the major themes and arguments. How the book proceeds. The skeleton. The authors have clearly stated that the book intends to help people understand expository works.

In simple terms the book is meant for people World health organization read serious Sachbuch. However, the authors have included sections on how to read fiction, plays and poetry as well. Stochern im nebel were formidable beasts that were Leid easily handled by strangers. Yet Aliena and Richard were able to Not only saddle the warhorse, but to get right on and ride it? I don’t think so.

He has carried that with him into this Narration. But it technisch an effective way to Donjon me looking over my shoulder. Along the way, you learn a Normale about medieval culture – particularly the role of Religion, the political Beherrschung of a monestary, priory, or diocese – how life is funded, and justament how much it sucks to be a serf. There’s dementsprechend quite a bit of focus on the reason for, and the means to, building cathedrals – Follett muses in his Foreward that one of the things he never could understand is why people in such destitute times would have put so much energy into buildings of such scale, and this book addresses that.

You im Folgenden learn a Senkrechte about architecture and the Entwicklung of cathedral-building. I can im weiteren Verlauf now tell you the difference between a nave, chancel, Querschiff, cloister, and clerestory. Oh, and probably 7 different words for “horse”. Coupled with this condescension is a Kind of willfully old-fashioned pretence.

My kombination Impression of the novel is that it is a Kurztrip de force of storytelling; a Narration that weaves together the lives of enemies and friends Who are Leid All completely evil or good, World health organization have their own dreams and ambitions, and Weltgesundheitsorganisation are willing to do dirty yet necessary things to achieve their ends. Some are Mora good than others, some are almost saintly, and others are steaming piles of doo.

But somehow, amazingly, they are All parties in the construction of this one cathedral, and the cathedral connects them in both death and life. In my experience, the Sauser common Fasson of this conversation is when an author quotes or alludes to another book in his own book.

The book quoted or alluded to is generally one that is considered a classic. Some instances can be fairly extensive as in Andrew Davidson’s. If this novel had been marketed as a revisionary narrative, it would have been in Ordnung. But it wasn’t. I’m justament glad that the historical aspect of the novel justament served as the Background and Leid the wirklich Geschichte. Because then, I probably would’ve stopped reading. The Bible translations is a prime example of it. But for me – and I’m assuming for a large number of other readers – it technisch.

Partie two: third Niveau, analytic reading 6 pigeonholing a book 7 x-raying a book 8 coming to terms with an author 9 determining author’s Aussage 10 criticizing a book fairly 11 agreeing or disagreeing with an author 12 Aids to reading. It im Folgenden hints at redemption. If something is this Badeort, it can be Made into something good once Mora; it has the Potenzial to be as it should be in the right hands.

I do love this Narration. It shows that if people can come together, to achieve something greater than themselves then humanity is Not Schwefellost despite the backdrop of hinter sich lassen, corruptness and General Unordnung. Of course, while reading it, I zur Frage making notes as usual – some were comments, others were highlights of the content.

Books can teach us something they can help us increase our understanding about the world although their authors may no longer be physically present. That’s great Berichterstattung, because that means that we have access to the greatest minds in the History of civilization!

Ken Follett describes a gloomy, dismal, nearly barbaric society whose citizens’ greatest concerns seem to be their egos and their Begierde for Herrschaft and control. The krank Who shared the Saatkorn dream as Tom Builder to have a cathedral rise up from the ashes of the old church at Kingsbridge. In fact, if it wasn’t for the translations, Traubenmost of Stochern im nebel books would never have enjoyed such a wide audience today. As discussed in the book, 6. An absolutely amazing reading experience.

I listened to the Audio Ausgabe of this book, which means just over 40 hours of content, and when it in dingen finished, I actually believed it should have been LONGER. This book grabbed me from the opening pages and kept me interested and engaged throughout the entire novel a very rare feet for a Diener novel. I can’t recommend this book highly enough.

In the second Distributionspolitik, you de rigueur judge whether what is being offered is really acceptable to you as knowledge. In the other words, there is oberste Dachkante the task of understanding the book, and second the Stellenanzeige of criticizing it. These two are quite separate, as you ist der Wurm drin See Mora and More. Vermutung are nachdem the four questions you ask when you are reading a book analytically. They are applicable to any Schriftart of book fiction or non-fiction , but when it comes to works of imaginative literature, like novels, poems, or plays, Vermutung four questions are altered a bit.

Having thousands of books behind me, I thought that a book with the title How to Read a Book would Fall into the Same category as the books about how to walk or how to ride a bicycle.

But then there’s a question — how many of those books I really understood and how many of them gerade gave me Auskunft, which would anyway be gone almost completely Anus a while?

This regards primarily to expository books, since reading of literature has a different purpose. Has anyone else noticed the numerous appearances of “his heart zum Thema in his mouth” or “with zu sich heart in zu sich mouth”? I would have thought an editor would have circled ONE use of such a cliche but I’m half way through and am at seven and counting.

Gripping Story though Against this richly imagined and intricately interwoven backdrop, filled with the ravages of Schluss machen mit and the rhythms of daily life, the master storyteller draws the reader irresistibly into the intertwined lives of his characters into their dreams, their labors, and their loves: Tom, the master builder; Aliena, the ravishingly beautiful noblewoman; Philip, the prior of Kingsbridge; Jack, the Artist in stone; and Ellen, the woman of the forest Weltgesundheitsorganisation casts a terrifying curse.

From humble stonemason to imperious Gebieter, each character is brought vividly to life. One idea from the book that I sprachlos recall 30 years later is his discussion about teachers, dead and alive. Non- detailed Listening: Identifying key terms, understanding concepts and interpreting the concepts both in speaking and writing.

Speaking: Formal oral presentations on topics from academic contexts – without the use of PPT slides. Reading: Reading for comprehension. Reading for Writing: Writing academic proposals- writing research articles: format and style. Grammar: Editing short texts — identifying and correcting common errors in grammar and usage articles, prepositions, tenses, subject verb agreement Pronunciation: Stress in compound words. Text Books: 1.

Detailed 2. Reference Books: 1. Bailey, Stephen. Academic writing: A handbook for international students. Routledge, Chase, Becky Tarver. Pathways: Listening, Speaking and Critical Thinking. Heinley ELT; 2nd Edition, Hewings, Martin. Cambridge Academic English B2. CUP, Students will become familiar with 2- dimensional and 3-dimensional coordinate systems L5. UNIT — V: Multiple integrals: 8 hrs Double and Triple integrals — Change of order of integration in double integrals — Change of variables to polar, cylindrical and spherical coordinates.

Applications: Finding Areas and Volumes. Graw Hill Education. Weir, Thomas calculus, 14thEdition, Pearson. Course Objectives: 1. To identify the importance of the optical phenomenon i. Understand the mechanism of emission of light, utilization of lasers as coherent light sources for low and high energy applications, study of propagation of light through optical fibers and their implications in optical communications.

Enlightenment of the concepts of Quantum Mechanics and to provide fundamentals of deBroglie matter waves, quantum mechanical wave equation and its application, the importance of free electron theory for metals and band theory for crystalline solids. Metals- Semiconductors-Insulators concepts utilization of transport phenomenon of charge carriers in semiconductors.

To explain the significant concepts of dielectric and magnetic materials that leads to potential applications in the emerging micro devices. To Understand the physics of Semiconductors and their working mechanism. To give an impetus on the subtle mechanism of superconductors using the concept of BCS theory and their fascinating applications.

Course Outcomes: 1. Explain the need of coherent sources and the conditions for sustained interference L2. Identify the applications of interference in engineering L3.

Analyze the differences between interference and diffraction with applications L4. Illustrate the concept of polarization of light and its applications L2. Classify ordinary refracted light and extraordinary refracted rays by their states of polarization L2 2. Explain various types of emission of radiation L2.

Identify the role of laser in engineering applications L3. Describe the construction and working principles of various types of lasers L1. Explain the working principle of optical fibers L2. Classify optical fibers based on refractive index profile and mode of propagation L2. Identify the applications of optical fibers in medical, communication and other fields L2. Apply the fiber optic concepts in various fields L3. Describe the dual nature of matter L1. Explain the significance of wave function L2.

Identify the role of classical and quantum free electron theory in the study of electrical conductivity L3. Classify the energy bands of solids L2.

Explain the concept of dielectric constant and polarization in dielectric materials L2. Summarize various types of polarization of dielectrics L2.

Interpret Lorentz field and Claussius-Mosotti relation in dielectrics L2. Classify the magnetic materials based on susceptibility and their temperature dependence L2. Explain the applications of dielectric and magnetic materials L2.

Apply the concept of magnetism to magnetic devices L3 5. Outline the properties of charge carriers in semiconductors L2. Identify the type of semiconductor using Hall effect L2. Identify applications of semiconductors in electronic devices L2. Diffraction: Introduction – Fresnel and Fraunhofer diffraction – Fraunhofer diffraction due to single slit, double slit – N-slits Qualitative — Diffraction Grating – Dispersive power and resolving power of Grating Qualitative.

Fiber optics: Introduction —Principle of optical fiber- Acceptance Angle – Numerical Aperture – Classification of optical fibers based on refractive index profile and modes — Propagation of electromagnetic wave through optical fibers – Applications. Free Electron Theory: Classical free electron theory Qualitative with discussion of merits and demerits — Quantum free electron theory— Equation for electrical conductivity based on quantum free electron theory- Fermi-Dirac distribution- Density of states 3D – Fermi energy.

Avadhanulu, P. Chand Publications, 11th Edition Bhattacharya and Poonam Tandon, Oxford press Applied Physics by P. Palanisamy SciTech publications.

Engineering Physics by M. Srinivasan, New Age international publishers Engineering Physics – Sanjay D. Jain, D. Sahasrabudhe and Girish, University Press 5. Pandey and S. Processor Commands: Processor Commands. Programming for Problem Solving, Behrouz A. Forouzan, Richard F. Kernighan, Dennis M. Ritchie, 2e, Pearson. Programming in C, Ashok N.

Kamthane, Amit Kamthane, Pearson. Troubleshooting of PC Hardware and Software issues. Awareness of cyber hygiene protecting the personal computer from getting infected with the viruses , worms and other cyber attacks. Task 1: Identification of the peripherals of a computer – Prepare a report containing the block diagram of the computer along with the configuration of each component and its functionality.

Students should get connected to their Local Area Network and access the Internet. Students customize their web browsers using bookmarks, search toolbars and pop up blockers.

Features to be covered:- Layouts, Inserting text objects, Editing text objects, Inserting Tables, Working with menu objects, Inserting pages, Hyper linking, Renaming, deleting, modifying pages, etc. Create a project certificate. Creating a scheduler 2. Calculating GPA 3. Calculating Total, average of marks in various subjects and ranks of students based on marks Features to be covered:- Format Cells, Summation, auto fill, Formatting Text, Cell Referencing, Formulae in excel, Charts, Renaming and Inserting worksheets, etc.

Task Demonstration and Practice of various features LaTeX — document preparation, presentation Features covered in Task 9 and Task 11 need to be explored in LaTex Task Tools for converting word to pdf and pdf to word.

Venkateswarlu, S. UNIT IV: Listening to short audio texts and identifying the context and specific pieces of information to answer a series of questions in speaking. English Phonetics for Indian Students- P. Bala Subramanian, Mac Millan Publications.

Determination of thickness of thin object by wedge method. Determination of wavelengths of different spectral lines in mercury spectrum using diffraction grating in normal incidence configuration. Determination of dispersive power of the prism. Determination of dielectric constant using charging and discharging method. Study the variation of B versus H by magnetizing the magnetic material B-H curve. Determination of numerical aperture and acceptance angle of an optical fiber.

Determination of wavelength of Laser light using diffraction grating. Determination of the resistivity of semiconductor by four probe method. To determine the energy gap of a semiconductor using p-n junction diode. Determination of Hall voltage and Hall coefficient of a given semiconductor using Hall Effect.

Measurement of resistance of a semiconductor with varying temperature. Balasubramanian, M. Exercise 1: 1. Write a C program to print a block F using hash , where the F has a height of six characters and width of five and four characters. Write a C program to compute the perimeter and area of a rectangle with a height of 7 inches and width of 5 inches. Write a C program to display multiple variables. Exercise 2: 1. Write a C program to calculate the distance between the two points.

Write a C program that accepts 4 integers p, q, r, s from the user where r and s are positive and p is even. If q is greater than r and s is greater than p and if the sum of r and s is greater than the sum of p and q print “Correct values”, otherwise print “Wrong values”. Exercise 3: 1. Write a C program to convert a string to a long integer. Write a program in C which is a Menu-Driven Program to compute the area of the various geometrical shape.

Write a C program to calculate the factorial of a given number. Exercise 4: 1. Write a program in C to display the n terms of even natural number and their sum. Write a program in C to display the n terms of harmonic series and their sum.

Write a C program to check whether a given number is an Armstrong number or not. Exercise 5: 1. Write a program in C to print all unique elements in an array.

Write a program in C to separate odd and even integers in separate arrays. Write a program in C to sort elements of array in ascending order. Write a program in C for multiplication of two square Matrices.

Write a program in C to find transpose of a given matrix. Exercise 7: 1. Write a program in C to search an element in a row wise and column wise sorted matrix. Write a program in C to print individual characters of string in reverse order. Exercise 8: 1. Write a program in C to compare two strings without using string library functions.

Write a program in C to copy one string to another string. Exercise 9: 1. Write a program in C to demonstrate how to handle the pointers in the program. Exercise 1. Write a program in C to add two numbers using pointers. Write a program in C to add numbers using call by reference. Write a program in C to find the largest element using Dynamic Memory Allocation. Write a program in C to swap elements using call by reference. Write a program in C to count the number of vowels and consonants in a string using a pointer.

Write a program in C to show how a function returning pointer. Write a C program to find sum of n elements entered by user. To perform this program, allocate memory dynamically using malloc function. To perform this program, allocate memory dynamically using calloc function.

Understand the difference between the above two programs 2. Write a program in C to convert decimal number to binary number using the function. Write a program in C to check whether a number is a prime number or not using the function. Write a program in C to get the largest element of an array using the function. Write a program in C to append multiple lines at the end of a text file. Write a program in C to copy a file in another name. Write a program in C to remove a file from the disk.

UNIT — I: Solving systems of linear equations, Eigen values and Eigen vectors: 10hrs Rank of a matrix by echelon form and normal form — Solving system of homogeneous and non- homogeneous linear equations — Gauss Elimination method — Eigenvalues and Eigen vectors and properties article Unit — II: Cayley—Hamilton theorem and Quadratic forms: 10hrs Cayley-Hamilton theorem without proof — Applications — Finding the inverse and power of a matrix by Cayley-Hamilton theorem — Reduction to Diagonal form — Quadratic forms and nature of the quadratic forms — Reduction of quadratic form to canonical forms by orthogonal transformation.

Singular values of a matrix, singular value decomposition text book Steven C. Jain, S. Iyengar and R. Understand the mechanism of corrosion and how it can be prevented. Advanced instrumental techniques are introduced. Plastics: Compounding, fabrication compression, injection, blown film and extrusion , preparation, properties and applications PVC, polycarbonates and Bakelite , mention some examples of plastic materials used in electronic gadgets, recycling of e-plastic waste waste to wealth.

Elastomers:- Introduction, preparation, properties and applications Buna S, thiokol and polyurethanes. Composite materials: Fiber reinforced plastics, conducting polymers, biodegradable polymers, biopolymers, biomedical polymers.

Corrosion:-Definition, theories of corrosion chemical and electrochemical , galvanic corrosion, differential aeration corrosion, stress corrosion, galvanic series, factors influencing rate of corrosion, corrosion control proper designing and cathodic protection , Protective coatings surface preparation, cathodic coatings, anodic coatings, electroplating and electroless plating [nickel] , Paints constituents, functions and special paints.

Jain and M. Latest edition. Murthy, P. The purpose of the course is to introduce principles of computer organization and the basic architectural concepts. Register Transfer language and microinstructions :Bus memory transfer, arithmetic and logical micro-operations,shift and rotate micro-operations Basic Computer Organization and Design:Stored program concept,computer Registers, common bus system,Computer instructions, Timing and Control, Instruction cycle, Memory Reference Instructions, Input—Output configuration and program Interrupt.

TextBooks: 1. Computer System Architecture,3rded. MorrisMano, PHI. Computer Organization, 5thed. Type conversions, Expressions, More about Data Output. Modules: Modules, Standard Modules, Packages. UNIT IV File Operations: Reading config files in python, Writing log files in python, Understanding read functions, read , readline and readlines , Understanding write functions, write and writelines , Manipulating file pointer using seek, Programming using file operations Object Oriented Programming: Concept of class, object and instances, Constructor, class attributes and destructors, Real time use of class in live projects, Inheritance , overlapping and overloading operators, Adding and retrieving dynamic attributes of classes, Programming using Oops support Design with Classes: Objects and Classes, Data modeling Examples, Case Study An ATM, Structuring Classes with Inheritance and Polymorphism.

Programming: Introduction to Programming Concepts with Scratch. Lambert, Cengage. Daniel Liang, Pearson. Time and Space complexity. Searching – Linear search, Binary search, Fibonacci search. Sorting- Insertion sort, Selection sort, Exchange Bubble sort, quick sort , distribution radix sort , merging Merge sort algorithms.

Reema Thareja, Oxford. Gilberg, Behrouz A. Forouzon, Cengage. Introduction to Chemistry laboratory — Molarity, normality, primary, secondary standard solutions, volumetric titrations, quantitative analysis 1. Determination of ferrous iron using standard K2Cr2O7 solution.

Determination of temporary and permanent hardness of water using standard EDTA solution. Determination of the concentration of acetic acid using sodium hydroxide pH-metry method. Determination of the concentration of strong acid vs strong base by conductometric method. Determination of strong acid vs strong base by potentiometric method. Determination of CaCO3 present in an egg shell. Estimation of Vitamin C.

Determination of phosphoric content in soft drinks. Adsorption of acetic acid by charcoal. Preparation of nylon-6, 6 and Bakelite demonstration only.

Of the above experiments at-least 10 assessment experiments should be completed in a semester. Outcomes: The students entering into the professional course have practically very little exposure to lab classes. The experiments introduce volumetric analysis; redox titrations with different indicators; EDTA titrations; then they are exposed to a few instrumental methods of chemical analysis. Thus at the end of the lab course, the student is exposed to different methods of chemical analysis and use of some commonly employed instruments.

They thus acquire some experimental skills. Reference Books 1. List of Experiments: 1 Write a program that asks the user for a weight in kilograms and converts it to pounds.

There are 2. Create variables called total and average that hold the sum and average of the three numbers and print out the values of total and average. Allow the user to specify how high the triangle should be. Ask the user to guess the number and print a message based on whether they get it right or not. The program should then check to see if the strings are of the same length. If they are of the same length, the program should alternate the characters of the two strings.

For instance, if the user enters , the output should be 1,, Write a program that asks the user for an algebraic expression and then inserts multiplication symbols where appropriate. Then find the longest run of zeros, the largest number of zeros in a row. For instance, the longest run of zeros in [1,0,1,1,0,0,0,0,1,0,0] is 4.

For instance, the list [1,1,2,3,4,3,0,0] would become [1,2,3,4,0]. The program should then give the user the option to convert from feet into inches, yards, miles, millimeters, centimeters, meters, or kilometers. Say if the user enters a 1, then the program converts to inches, if they enter a 2, then the program converts to yards, etc. While this can be done with if statements,it is much shorter with lists and it is also easier to add new conversions if you use lists.

If the strings are identical, it should return In the function definition, set the default value of n to 2. Let the default value of n be Your program should print out a string consisting of those email addresses separated by semicolons. The possible units are inches, feet, yards, miles, kilometers, meters, centimeters, and millimeters. For each of these units there should be a method that returns the length converted into those units. For example, using the Converter object created above, the user could call c.

The program then displays the contents of the file in a textbox. Course Objectives: The objective of this lab is to demonstrate the different data structures implementation. Exercise -1 Searching a Write C program that use both recursive and non recursive functions to perform Linear search for a Key value in a given list. Exercise -2 Sorting-I a Write C program that implement Bubble sort, to sort a given list of integers in ascending order b Write C program that implement Quick sort, to sort a given list of integers in ascending order c Write C program that implement Insertion sort, to sort a given list of integers in ascending order.

Exercise -3 Sorting-II a Write C program that implement radix sort, to sort a given list of integers in ascending order b Write C program that implement merge sort, to sort a given list of integers in ascending order. Exercise -4 Singly Linked List a Write a C program that uses functions to create a singly linked list b Write a C program that uses functions to perform insertion operation on a singly linked list c Write a C program that uses functions to perform deletion operation on a singly linked list d Write a C program to reverse elements of a single linked list.

Exercise -5 Queue a Write C program that implement Queue its operations using arrays. Exercise -6 Stack a Write C program that implement stack its operations using arrays b Write C program that implement stack its operations using Linked list c Write a C program that uses Stack operations to evaluate postfix expression.

Exercise -7 Binary Tree d Write a recursive C program for traversing a binary tree in preorder, inorder and postorder. Role of information technology in environment and human health. Ecosystems: Concept of an ecosystem. Forest resources: Use and over — exploitation, deforestation — Timber extraction — Mining, dams and other effects on forest and tribal people. Water resources: Use and over utilization of surface and ground water — Floods, drought, conflicts over water, dams — benefits and problems.

Mineral resources: Use and exploitation, environmental effects of extracting and using mineral resources. Food resources: World food problems, changes caused by non-agriculture activities-effects of modern agriculture, fertilizer-pesticide problems, water logging, salinity.

Energy resources: Growing energy needs, renewable and non-renewable energy sources use of alternate energy sources. Land resources: Land as a resource, land degradation, Wasteland reclamation, man induced landslides, soil erosion and desertification; Role of an individual in conservation of natural resources; Equitable use of resources for sustainable lifestyles.

UNIT III Biodiversity and its conservation: Definition: genetic, species and ecosystem diversity- classification – Value of biodiversity: consumptive use, productive use, social-Biodiversity at national and local levels. India as a mega-diversity nation – Hot-sports of biodiversity – Threats to biodiversity: habitat loss, man-wildlife conflicts. Role of an individual in prevention of pollution. Impact of Fire Crackers on Men and his well being.

Solid Waste Management: Sources, Classification, effects and control measures of urban and industrial solid wastes. Consumerism and waste products, Biomedical, Hazardous and e — waste management. UNIT V Social Issues and the Environment: Urban problems related to energy -Water conservation, rain water harvesting-Resettlement and rehabilitation of people; its problems and concerns.

Environmental ethics: Issues and possible solutions. Ecotourism, Green Campus — Green business and Green politics. Text Books: 1 Environmental Studies, K.

 
 

Corel pdf fusion xps stack buffer overflow vulnerability free. Corel PDF Fusion XPS Stack Buffer Overflow (CVE-2013-3248)

 
 

Директор АНБ напоминал тигра на привязи. Лицо его все сильнее заливалось краской. – Невероятно! – воскликнул он и снова швырнул трубку.